ʻIke Hoʻohui ʻia nā Māka Ola Canine (5-6 mau mea)

【 Ke kumu hoʻāʻo 】
Canine pancreatic lipase (cPL): ʻO ka canine pancreatitis he maʻi infiltrative inflammatory o ka pancreas. ʻO ka maʻamau, hiki ke hoʻokaʻawale ʻia i ka pancreatitis acute a me ka pancreatitis maʻi. Hiki ke ʻike ʻia ka infiltration neutrophil pancreatic, pancreatic necrosis, peripancreatic fat necrosis, edema a me ka hōʻeha i ka maʻi pancreatitis. Hiki ke ʻike ʻia ka fibrosis pancreatic a me ka atrophy i ka pancreatitis mau. Ke hoʻohālikelike ʻia me ka maʻi maʻi pancreatitis, ʻoi aku ka maikaʻi o ka maʻi pancreatitis, akā ʻoi aku ka pinepine. Ke loaʻa nā ʻīlio i ka pancreatitis, ua pōʻino ka pancreas, a piʻi nui ka pae o ka pancreatic lipase i loko o ke koko. I kēia manawa, ʻo ka pancreatic lipase kekahi o nā hōʻailona maikaʻi loa o ka kikoʻī no ka maʻi o ka pancreatitis i nā ʻīlio.
ʻO Cholyglycine (CG) kekahi o nā waikawa cholic conjugated i hoʻokumu ʻia e ka hui pū ʻana o ka cholic acid a me ka glycine. ʻO ka waikawa Glycocholic ka mea nui loa o ka waikawa bile i ka serum i ka wā o ka hāpai ʻana. I ka wā i hōʻino ʻia ai nā pūnaewele ate, ua emi iho ka lawe ʻana o CG e nā puʻupaʻa o ke akepaʻa, a ma muli o ka hoʻonui ʻana o ka maʻiʻo CG i loko o ke koko. I ka cholestasis, ua hoʻopilikia ʻia ka excretion o ka waika cholic e ke akepaʻa, a ua hoʻonui ʻia ka ʻike o CG i ke kahe koko, e hoʻonui ai i ka ʻike o CG i loko o ke koko.
ʻO Cystatin C kekahi o nā protein cystatin. I kēia manawa, ʻo Cys C kahi mea endogenous e kū pono ana i nā koi o kahi hōʻailona GFR endogenous kūpono. He index sensitive a kikoʻī no ka loiloi o ka hana renal canine.
ʻO ka N-terminal pro-brain natriuretic peptide (Canine NT-proBNP) kahi mea i huna ʻia e nā cardiomyocytes i loko o ka Canine ventricle a hiki ke hoʻohana ʻia ma ke ʻano he kuhikuhi ʻike no ka hāʻule ʻole o ka naʻau. Hoʻopili ka ʻike o ka cNT-proBNP i ke koko me ka paʻakikī o ka maʻi. No laila, ʻaʻole hiki i ka NT-proBNP ke loiloi wale i ke koʻikoʻi o ka puʻuwai puʻuwai a me ka maʻi maʻamau, akā hoʻohana ʻia hoʻi i mea hōʻailona o kāna prognosis.
Canine allergen total IgE (cTIgE): He ʻano immunoglobulin (Ig) ʻo IgE me ke kaumaha molekala o 188kD a he haʻahaʻa haʻahaʻa loa i loko o ka serum. Hoʻohana maʻamau ia no ka ʻike ʻana i nā hopena allergy. Eia hou, hiki ke kōkua i ka ʻike ʻana i nā maʻi parasitic a me ka myeloma lehulehu. 1. Ka hopena ma'i: ke hiki mai ka ma'i ma'i, alaka'i ia i ka pi'i 'ana o ka allergen lgE. ʻO ke kiʻekiʻe o ka allergen lgE, ʻoi aku ka koʻikoʻi o ka hopena maʻi. 2. ʻO ka maʻi parasite: ma hope o ka loaʻa ʻana o ka pipi e nā parasite, hiki ke piʻi aʻe ka allergen lgE, ʻo ia hoʻi e pili ana i ka maʻi maʻi maʻi i hoʻokumu ʻia e nā protein parasite. Eia kekahi, ʻo ka loaʻa ʻana o ka maʻi kanesa hiki ke kōkua i ka piʻi ʻana o ka IgE holoʻokoʻa.

【 Kuleana ʻike 】
Hoʻohana kēia huahana i ka immunochromatography fluorescence e ʻike nui i ka ʻike cPL/CG/cCysC/cNT-proBNP/cTIgE i loko o ke koko canine. ʻO ke kumu kumu, ua kaha ʻia ka membrane nitrocellulose me nā laina T a me C, a ua uhi ʻia ka laina T me ka antibody a e ʻike pono i ka antigen. Hoʻopili ʻia ka pā paʻa me kekahi nanomaterial fluorescent i kapa ʻia ka antibody b hiki ke ʻike pono i ka antigen. Hoʻopili ka antibody i loko o ka laʻana i ka nanomaterial i kapa ʻia ʻo antibody b e hana i kahi paʻakikī, a laila hoʻopaʻa i ka antibody T-laina A e hana i kahi ʻano hana sandwich. Ke hoʻomālamalama ʻia ke kukui hauʻoli, hoʻopuka ka nanomaterial i nā hōʻailona fluorescent. Ua hoʻopili maikaʻi ʻia ka ikaika o ka hōʻailona me ka ʻike antigen i ka hāpana.