Feline Health Marker Combined Detection（5-6 mau mea）

【 Ke kumu hoʻāʻo 】
ʻO Feline pancreatic lipase (fPL): ʻO ka pancreas ka lua o ka nui o ka digestive gland i loko o ke kino holoholona (ʻo ka mua ka ate), aia ma ka ʻōpū mua o ke kino, i māhele ʻia i nā lobes hema a ʻākau. ʻO kāna hana nui ka huna ʻana i nā enzyme e pono ai no ke kino. Ua māhele ʻia ka maʻi pancreatitis i ka maʻi pancreatitis a me ka pancreatitis maʻi. ʻO ka pōʻino i hana ʻia e ka mea mua he manawa pōkole wale nō, aʻo ka mea hope e waiho i ka fibrosis mau a me ka atrophy i ka wā o ka ʻeha mau. Ma waena o lākou, ʻo ka maʻi maʻi pancreatitis e pili ana i ka 2/3 o ka maʻi pancreatitis.
ʻO Cholyglycine (CG) kekahi o nā waikawa cholic conjugated i hoʻokumu ʻia e ka hui pū ʻana o ka cholic acid a me ka glycine. ʻO ka waikawa Glycocholic ka mea nui loa o ka waikawa bile i ka serum i ka wā o ka hāpai ʻana. I ka wā i hōʻino ʻia ai nā pūnaewele ate, ua emi iho ka lawe ʻana o CG e nā puʻupaʻa o ke akepaʻa, a ma muli o ka hoʻonui ʻana o ka maʻiʻo CG i loko o ke koko. I loko o ka cholestasis, ua hoʻopilikia ʻia ka excretion o ka cholic acid e ke akepaʻa, a ua hoʻonui ʻia ka ʻike o ka CG i ke kahe o ke koko, kahi e hoʻonui ai i ka ʻike o ka CG i loko o ke koko. ma ka hepatic duct ma hope o ka ʻai ʻana. Pēlā nō, hiki i nā maʻi o ka ate a me ka ʻākea ʻana o ka bile duct ke kumu i ka ʻōlelo kuhikuhi abnormal.
ʻO Cystatin C kekahi o nā protein cystatin. ʻO ka hana koʻikoʻi koʻikoʻi ka hoʻoponopono ʻana i ka hana o ka cysteine ​​​​protease, ʻo ia ka mea i loaʻa ka hopena inhibitory ikaika loa ma cathepsin B, papain, figs protease, a me cathepsin H a me I hoʻokuʻu ʻia e lysosomes. He kuleana koʻikoʻi ia i ka metabolism o nā peptides intracellular a me nā protein, ʻoi aku hoʻi i ka metabolism o ka collagen, hiki iā ia ke hydrolyze i kekahi mau prehormones a hoʻokuʻu iā lākou i loko o nā kiko kikoʻī e pāʻani i kā lākou mau kuleana biological. ʻO ka maʻi hemorrhage hereditary me amyloidosis he maʻi e pili pono ana i ka mutation gene cystatin C, hiki ke hoʻoulu i ka cerebral vascular rupture, cerebral hemorrhage a me nā hopena koʻikoʻi ʻē aʻe. ʻO ka puʻupaʻa wale nō kahi e hoʻomaʻemaʻe ai i ka cystatin C, a ʻo ka hana mau ʻana o ka cystatin C. ʻO ka pae serum cystatin C e hilinaʻi nui ʻia i ka GFR, kahi hōʻailona endogenous kūpono e hōʻike i nā loli o GFR. ʻO ka hoʻololi ʻana i ka ʻike o nā wai kino ʻē aʻe e pili pū ana me nā maʻi like ʻole.
ʻO NT-proBNP (N-terminal pro-brain natriuretic peptide), i kapa ʻia ʻo B-type diuretic peptide, he hormone protein i huna ʻia e nā cardiomyocytes i loko o nā ventricle o ka puʻuwai. Ke piʻi aʻe ke koko ventricular, hoʻonui ʻia ka ventricular dilation, myocardial hypertrophy, a i ʻole ke kaomi ʻana i ka myocardium, ua hūnā ʻia ka precursor o NT-proBNP, proBNP (he 108 amino acids), i loko o ke kahe koko e nā cardiomyocytes.
Cat allergen total IgE (fTIgE): ʻO IgE kekahi ʻano immunoglobulin (Ig) me ke kaumaha molekala o 188kD a me kahi mea haʻahaʻa loa i loko o ka serum. Hoʻohana maʻamau ia no ka ʻike ʻana i nā hopena allergy. Eia hou, hiki ke kōkua i ka ʻike ʻana i nā maʻi parasitic a me ka myeloma lehulehu. 1. Ka hopena ma'i: ke hiki mai ka ma'i ma'i, alaka'i ia i ka pi'i 'ana o ka allergen lgE. ʻO ke kiʻekiʻe o ka allergen lgE, ʻoi aku ka koʻikoʻi o ka hopena maʻi. 2. ʻO ka maʻi parasite: ma hope o ka loaʻa ʻana o ka pipi e nā parasite, hiki ke piʻi aʻe ka allergen lgE, ʻo ia hoʻi e pili ana i ka maʻi maʻi maʻi i hoʻokumu ʻia e nā protein parasite. Eia kekahi, ʻo ka loaʻa ʻana o ka maʻi kanesa hiki ke kōkua i ka piʻi ʻana o ka IgE holoʻokoʻa.
【 Kuleana ʻike 】
Hoʻohana kēia huahana i ka immunochromatography fluorescence e ʻike nui i ka ʻike o ka fPL/CG/fCysC/fNT-proBNP/fTIgE i loko o ke koko pōpoki. ʻO ke kumu kumu, ua kaha ʻia ka membrane nitrocellulose me nā laina T a me C, a ua uhi ʻia ka laina T me ka antibody a e ʻike pono i ka antigen. Hoʻopili ʻia ka pā paʻa me kekahi nanomaterial fluorescent i kapa ʻia ka antibody b hiki ke ʻike pono i ka antigen. Hoʻopili ka antibody i loko o ka laʻana i ka nanomaterial i kapa ʻia ʻo antibody b e hana i kahi paʻakikī, a laila hoʻopaʻa i ka antibody T-laina A e hana i kahi ʻano hana sandwich. Ke hoʻomālamalama ʻia ke kukui hauʻoli, hoʻopuka ka nanomaterial i nā hōʻailona fluorescent. Ua hoʻopili maikaʻi ʻia ka ikaika o ka hōʻailona me ka ʻike antigen i ka hāpana.